PIAS3 polyclonal antibody
产品名称： PIAS3 polyclonal antibody
英文名称： PIAS3 polyclonal antibody
产品编号： PAB1853
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PIAS3.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PIAS3.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- PIAS3 interacts with ATF1 and regulates the human ferritin H gene through an antioxidant-responsive element.
Iwasaki K, Hailemariam K, Tsuji Y.J Biol Chem. 2007 Aug 3;282(31):22335-43. Epub 2007 Jun 12.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of PIAS3 polyclonal antibody (Cat # PAB1853) in HL-60 cell lysate. PIAS3 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with PIAS3 polyclonal antibody (Cat # PAB1853) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 10401
- Protein Accession#:
- NP_006090;Q9Y6X2
- Gene Name:
- PIAS3
- Gene Alias:
- FLJ14651,ZMIZ5
- Gene Description:
- protein inhibitor of activated STAT, 3
- Omim ID:
- 605987
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq
- Other Designations:
- OTTHUMP00000015586,zinc finger, MIZ-type containing 5